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LIFA-X Fluorescence and Phosphorescence Lifetime Imaging

作者: Kevin Peng    发布于: 2014-07-01 00:41    点击:

This newest addition to the LIFA product line offers fluorescence lifetime imaging microscopy in the range from 0ns up to 1 ms (Phosphorescence). The 'X' represents the extended range of lifetimes that can be resolved when compared to the standard LIFA (0.1 ns - 300 ns).

You can see the LIFA-X at one of our Test Centers.


 

Description

This newest addition to the LIFA product line offers fluorescence lifetime imaging microscopy in the range from 0ns up to 1 ms (Phosphorescence). The 'X' represents the extended range of lifetimes that can be resolved when compared to the standard LIFA (0.1 ns - 300 ns). The LIFA-X is the ideal solution for:

  • Oxygen sensing of biofilms, tissues and cells
  • Protein-protein dynamics through luminescence resonance energy transfer (LRET), e.g. Tryptophan phosphorescence
  • Protein folding and protein conformation
  • Time-gated luminescence imaging (NEW!)

The LIFA-X is compatible with Leica, Nikon, Olympus and Zeiss widefield fluorescence microscopes. The TRiCAM-X modulated intensified CCD camera can also be retro-fitted onto an existing LIFA system. Please read our LIFA-X application notes for more technical information.

Please view our list of LIFA reference customers.

Features

  • Fast fluorescence lifetime imaging microscopy, up to two lifetime images per second
  • Non-phototoxic illumination offered by the Multi-LED (NEW)
  • Lifetime sensitivity 0 ns - 1 ms
  • High quantum efficiency with the Gen III GaAs intensifier (Optional)

Key analysis routines

  • Time-lapses
  • Multi-frequency acquisition & analysis for separation of multiple lifetimes
  • Polar (Phasor) plot inspection and separation of multiple lifetimes

Options

Image intensifiers

The LIFA camera can be produced with different image intensifiers for different spectral sensitivity. Choose the image intensifier suitable for your application here.

Support for automated microscopes

Nikon Ti Eclipse.

Support for XYZ stages

Other

Leica, Nikon and Olympus TIRF.

Applications

  • Molecular interactions
  • Protein conformation
  • Biosensors
  • Oxygen concentration imaging in cells and tissue
  • NADH / FAD fluorescence dynamics
  • Viscosity imaging
  • Membrane dynamics
  • Membrane trafficking
  • Crude oil characterization
  • LED inspection
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