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LIFA Hyperspectral

作者: Kevin Peng    发布于: 2014-07-01 00:38    点击:

The LIFA is a camera-based fluorescence lifetime imaging microscopy system that can be easily combined with the HSi hyperspectral unit of Gooch & Housego. Acquiring lifetimes at a range of wavelengths opens up the possibility to spectrally resolve multi-component lifetime distributions into their lifetime components.

 



 

Description

The LIFA is a camera-based fluorescence lifetime imaging microscopy system that can be easily combined with the HSi hyperspectral unit of Gooch & Housego. Acquiring lifetimes at a range of wavelengths opens up the possibility to spectrally resolve multi-component lifetime distributions into their lifetime components.

Moreover spectrally resolved fluorescence lifetime imaging microscopy allows one to track both the donor and acceptor molecules in FRET experiments, and enables the observation of a range of spectroscopic changes invisible to a standard fluorescence lifetime imaging microscopy system. The addition of polarizers further enables the detailed anisotropy characterisation required in diffusion studies.

The widefield configuration includes the new Multi-LED modulated light source. Its high-power LEDs can be modulated in a broad frequency range, resulting in good lifetime sensitivity and high accuracy.

The LIFA is compatible with Leica, Nikon, Olympus, TILL and Zeiss fluorescence microscopes.

Features

Hardware features

  • Fast fluorescence lifetime imaging microscopy, up to two lifetime images per second
  • Non-phototoxic illumination offered by the Multi-LED (NEW)
  • Lifetime sensitivity 0-300 ns
  • Lifetime accuracy 30 ps r.m.s.
  • High quantum efficiency with the optional Gen III GaAs intensifier

Software features

  • Full control of the HSi unit from LI-FLIM
  • FRET efficiency mapping
  • Polar (Phasor) plot inspection and separation of multiple lifetimes
  • Export to Metamorph, ImageJ, Matlab
  • Images can be exported as BMP, TIFF
  • Statistics and histograms can be exported as MS Excel files

Models

Lambert Instruments provides the following LIFA models, which cover a wide range in spectral sensitivity and in lifetime sensitivity. Each model is based on a specific high-resolution Gen II or Gen III image intensifier, see also our fluorescence lifetime imaging microscopy properties of modulated intensifiers document.

Model

Photocathode

Modulation frequency

Lifetime sensitivity

LIFA Gen II S20 S20 1-120 MHz 0-300 ns
LIFA Gen II S25 S25 1-120 MHz 0-300 ns
LIFA Gen III GaAs GaAs 1-120 MHz 0-300 ns
LIFA X Gen II S20 S20 0-100 kHz, 1-120 MHz 0 ns - 1 ms
LIFA X Gen II S25 S25 0-100 kHz, 1-120 MHz 0 ns - 1 ms
LIFA X Gen III GaAs GaAs 0-100 kHz, 1-120 MHz 0 ns - 1 ms
LIFA P Gen II S20 S20 0-100 kHz 100 ns - 1 ms
LIFA P Gen II S25 S25 0-100 kHz 100 ns - 1 ms
LIFA P Gen III GaAs GaAs 0-100 kHz 100 ns - 1 ms
LIFA P Gen III GaAsP GaAsP 0-100 kHz 100 ns - 1 ms

 

Photocathode spectral sensitivity

Spectral sensivity as a function of wavelength for each of the photocathodes is shown below.

 

For more information, please refer to our technologies page on the operating principle of image intensifiers for lifetime imaging.

Options

Support for automated microscopes

Nikon Ti Eclipse.

Support for XYZ stages

Applications

The LIFA has the following applications:

  • Molecular interactions
  • Protein conformation
  • Biosensors
  • Oxygen concentration imaging in cells and tissue
  • NADH/FAD fluorescence dynamics
  • Viscosity imaging
  • Membrane dynamics
  • Membrane trafficking
  • LED inspection
  • Crude oil characterisation
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